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KMID : 0613820130230070869
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Journal of Life Science
2013 Volume.23 No. 7 p.869 ~ p.878
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Antioxidant and Anti-inflammatory Activities of Functional Plant Materials
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Lee Soo-Jung
Shin Jung-Hye
Lee Hye-Jin
Tak Hyun-Min
Kang Min-Jung
Sung Nak-Ju
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Abstract
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Eleven functional plant materials were identified via a literature search, and their antioxidant capacity and inhibitory effects on lipopolysaccharide (LPS)-induced nitric oxide (NO) production in RAW264.7 cells were tested. Yields from hot water extracts of the materials were the highest (52.10%) in Lycii fructus, and the yields from Phellinus linteus were the lowest (5.7%). The yields of another were 14.50-42.47%. Total phenol and flavonoids contents were the highest in P. linteus. The EC_50 values for DPPH and ABTS radical scavenging activities were lower than 100μg/ml for Salvia miltiorrhiza, whereas the values for P. linteus, Scutellaria baicalensis, and Paeonia lactiflora were 100-200μg/ml. The EC_50 value for the superoxide anion radical scavenging activity of all the extracts was higher than 300μg/ml. P. linteus for the reducing power was shown the highest activity. Fe²+ chelating activity was the highest in the Morus alba extract. In an MTT assay, the cell viability of the RAW264.7 LPS-exposed cells was above 80% in extracts of 50μg/ml and above 77% in extracts of 100μg/ml in all the plant materials except Acanthopanax sessiliflorum. NO production in the RAW264.7 LPS-exposed cells showed a 12-fold increase compared to the control. The NO production level of all the extracts was 6.86~26.18μM. Notably, 100μg/ml of S. baicalensis extract showed a remarkable decrease in NO production (72%) compared with the control. The potent antioxidant and anti-inflammatory activities of S.baicalensis, P. linteus, S. miltiorrhiza, M. alba, and P. lactiflora suggest that they are potential candidates as functional materials.
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KEYWORD
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Antioxidant, functional plant materials, immune activity, NO production
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